Images showed around 250 farmers, lying down on bails of hay as they blocked part of the famous avenue in Paris from before sunrise.
Paris police advised motorists to stay away from the Concorde / Champs-Elysées sector. Riot police surrounded the farmers and their reports of tensions rising between the two groups.
"Macron is killing farmers" was one of the slogans they shouted hoping to "wake up" the president to the plight. Whilesome farmers protested, another delegation was due to meet Macron at the Elysée Palace.
Farmers in France regularly stage protest stunts on the capital's most famous avenue and Friday's action was in protest against Macron's desire to ban the use of glyphosate - a herbicide that has been linked to health dangers including cancer.
The herbicide is the mostly commonly used in Europe, but Macron has signalled his intention to seek a ban on the product, which is an ingredient of Round-up, the weedkiller sold by Monsanto.
The EU has suggested renewing the authorization for glyphosate for a further 10 years but a vote will take place in October and France has said it will oppose the move.
"Mr Macron wants to be the the world's Mr Clean but he is the gravedigger of agriculture," said Frédéric Arnoult, president of Young Farmers union.
"How can we want to sell the dream to the French of a healthy diet, while they take away our means of production?", he asked.