In the speech she denounced the holding of Muslim prayers in the streets of France - where a dearth of mosques had forced many to pray outside - saying: "For those who like to talk about World War II, to talk about occupation, we could talk about, for once, the occupation of our territory."
"It is an occupation of part of the territory, suburbs where religious law is applied. Sure, there are no armoured vehicles, no soldiers, but it is an occupation nonetheless and it weighs on residents."
The move to put her on trial comes after the European Parliament has stripped the National Front leader of her immunity as a lawmaker in July 2013.
That decision left her open to face prosecution in France, but on Tuesday the court in Lyon cleared her of all charges.
While relieved of these charges, Le Pen is still facing legal woes.
Her party has also been slapped with charges of fraud as part of an ongoing probe into campaign financing.